Enzymes and Inhibitors
MP Biomedicals™ Phorbol-12-myristate-13-acetate, >99%
Phorbol-12-myristate-13-acetate, >99% is a potent tumor promoter and activates protein kinase C in vivo and in vitro.
Thermo Scientific™ TscAI (TspRI) (10 U/μL)
Cut at CASTG(2/-7)^ sites with TscAI (TspRI) restriction enzyme, which functions best at 65°C in Tango buffer (Isoschizomers: TspRI).
Invitrogen™ TrueCut™ HiFi Cas9 Protein (1 μg/μL)
A high fidelity CRISPR Cas9 protein variant engineered to demonstrate superior off-target profiles, while preserving maximum on-target editing efficiency. TrueCut™ HiFi Cas9 Protein is ideal for applications that are sensitive to off-target events and when more precise editing is required.
Thermo Scientific™ Bsh1236I (BstUI) (10 U/μL)
Cut at CG^CG sites with Bsh1236I (BstUI) restriction enzyme, which performs best at 37°C in R buffer (Isoschizomers: AccII, BspFNI, BstFNI, BstUI, MvnI).
Thermo Scientific™ Psp1406I (AclI) (10 U/μL)
The Psp1406I (AclI) restriction enzyme recognizes AA^CGTT sites and cuts best at 37°C in Tango buffer (Isoschizomers: AclI).
R&D Systems™ Pan Caspase Inhibitor Z-VKD-FMK Biotinylated
Used as an intracellular detector of apoptotic cells. R&D Systems Caspase-13 Inhibitor Z-LEED-FMK binds to the active site of activated proteases irreversibly and inhibits apoptosis.
Thermo Scientific™ BseGI (BtsCI) (10 U/μL)
Cut at GGATG(2/0)^ sites with BseGI (BtsCI) restriction enzyme, which performs best at 55°C in Tango buffer (Isoschizomers: BstF5I, BtsCI, FokI).
MilliporeSigma™ Calbiochem™ FragEL™ TdT Enzyme DNA Fragmentation Detection Kit, Colorimetric
For detection of apoptosis at individual cell level
Cytiva M13KO7 Helper Phage
Used for phage rescue. Cytiva M13KO7 Helper Phage is used in fluorescence quantification of single stranded M113DNA.
MP Biomedicals™ BCIP/NBT Liq. Substrate Plus, 100mL
Stabilized chromogen solution with greater sensitivity and produces little or no background staining compared with other alternatives.
Invitrogen™ DDAO galactoside (9H-(1,3-Dichloro-9,9-Dimethylacridin-2-One-7-yl) β-D-Galactopyranoside)
The galactosidase substrate, DDAO galactoside yields a hydrolysis product that can be excited with the 633 nm laser (excitation/emission maxima ∼645/660).
R&D Systems™ Recombinant Human BACE-2 Protein
Extensive quality control produces industry leading bioactivity and lot-to-lot consistency that instills confidence in results and ensures reproducibility. Applications: Enzyme Activity
StatLab MasterTech Diastase
StatLab Diastase, a MasterTech Special Stain Kit Component